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rabbit α human eif4e  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit α human eif4e
    Rabbit α Human Eif4e, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+%CE%B1+human+eif4e/bio_rxiv__2025__10__04__680434-220-41-44
    Average 86 stars, based on 1 article reviews
    rabbit α human eif4e - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    SDS Page:

    Article Title: Structural and mechanistic insights into translation initiation on the enterovirus Type 1 IRES
    Article Snippet: Eluate was adjusted to a KCl concentration of 100 mM before application to a Capto HiRes S 10/100 column (Cytiva) equilibrated in buffer D. A linear KCl gradient from 100 mM to 1.0 M (30 CV) was used to elute eIF3-containing fractions, which were pooled and layered onto a 10-30% (w/v) sucrose gradient in buffer E (20 mM Tris-HCl pH 7.5, 400 mM KCl, 2.0 mM DTT, 0.1 mM EDTA, 10% w/v glycerol). .. Ultracentrifugation in an SW-41 rotor (287,000 x g, 4°C, 22 h) was performed to separate eIF3 from eIF4G, as determined by SDS-PAGE and Western blotting of fractions with rabbit α-human eIF3D (Proteintech, 10219-1-AP), rabbit α-human eIF4G (Cell Signalling Technology, C45A4) and rabbit α-human eIF4E (Cell Signalling Technology, C46H6) antibodies. .. Suitable fractions were exchanged into buffer D and applied to a MonoQ 5/50 anion exchanger (Cytiva) equilibrated in buffer D. A linear gradient from 100 mM → 500 mM KCl (20 CV) was used to elute eIF3.

    Western Blot:

    Article Title: Structural and mechanistic insights into translation initiation on the enterovirus Type 1 IRES
    Article Snippet: Eluate was adjusted to a KCl concentration of 100 mM before application to a Capto HiRes S 10/100 column (Cytiva) equilibrated in buffer D. A linear KCl gradient from 100 mM to 1.0 M (30 CV) was used to elute eIF3-containing fractions, which were pooled and layered onto a 10-30% (w/v) sucrose gradient in buffer E (20 mM Tris-HCl pH 7.5, 400 mM KCl, 2.0 mM DTT, 0.1 mM EDTA, 10% w/v glycerol). .. Ultracentrifugation in an SW-41 rotor (287,000 x g, 4°C, 22 h) was performed to separate eIF3 from eIF4G, as determined by SDS-PAGE and Western blotting of fractions with rabbit α-human eIF3D (Proteintech, 10219-1-AP), rabbit α-human eIF4G (Cell Signalling Technology, C45A4) and rabbit α-human eIF4E (Cell Signalling Technology, C46H6) antibodies. .. Suitable fractions were exchanged into buffer D and applied to a MonoQ 5/50 anion exchanger (Cytiva) equilibrated in buffer D. A linear gradient from 100 mM → 500 mM KCl (20 CV) was used to elute eIF3.



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    86
    Cell Signaling Technology Inc rabbit α human eif4e
    Rabbit α Human Eif4e, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+%CE%B1+human+eif4e/bio_rxiv__2025__10__04__680434-220-41-44
    Average 86 stars, based on 1 article reviews
    rabbit α human eif4e - by Bioz Stars, 2026-10
    86/100 stars
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